Tools of Biotechnology-III


4362 bsae pairs
Tet. and Amp. resistant
20  - 50 copies per cell

Vectors for DNA delivery
l Plasmids are the extrachromosomal DNA, present in the bacterial cells.
l These are a). Small’ b). Circular, c). self replicating and d). can mobilize from one cell to another.
l Vectors are derived from the natural plasmids, by making them a). smaller b). drug resistant c). relaxed control of replication and d). convenient restriction sites etc, by genetic manipulation
l Plasmid vector pBR 322 is a typical example that has been used for many of the important discoveries in Biotechnology

 

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