IISR was established in the year 1952 with the objective of doing basic and strategic research in increasing sugarcane yield, train personnel at various levels and Poster international and departmental linkages.

Dr. R. L. Yadav
Indian Institute of Sugarcane Research,
Raibareli Road
P.O. Dilkusha, Lucknow-226 002
UP, INDIA
Phone: +91-522-2480735 / 7
Fax: +91-522-2480738
E-mail : IISRLko@sancharnet.in , IISR_Lko@sancharnet.in
Website: http://www.upgov.up.nic.in/iisr

• Molecular characterization of red rot pathogen: Cloned DNA probes for the detection of incipient infection of major pathogens in sugarcane. Identification of molecular markers linked sucrose synthesis and inversion for increasing sucrose content in sugarcane.
• DNA Finger printing of sugarcane genotypes of subtropical india: Work is also underway to identify Saccharum species and hybrids using fluorescent in situ hybridization for physical localization of rRNA gene families on the chromosomes. Work is also underway to identify Saccharum species and hybrids using fluorescent in situ hybridization for physical localization of rRNA gene families on the chromosomes. 
• Genetic transformation of sugarcane: This Institute is engaged in genetic transformation of sugarcane for conferring resistance to biotic and abiotic stresses. Electroporation and Agrobacterium mediated transformation of sugarcane varieties is being done to introduce Bacillus thuringiensis cry genes into sugarcane. Transgenic sugarcane is being raised by employing GUS reporter gene through electroporation.
• Development of bio fertilizers: The endophytic bacteria of sugarcane Acetobacter have been analysed by RAPD to identify linkages.

Major Equipments and Facilities:

• Thermal Cycler 
• Gel Documentation unit 
• High Speed Refrigerated Centrifuge 
• Electro Cell Manipulator 
• Electrophoresis Units 
• Tissue Culture facility for transformation 

Computer & Networking

• Operating System - SOLARIS, Novell 4.1 Win 35/95/98/2000
• Number of Nodes - 100 +
• Softwares - Word star 4.0, dBASE, III +, LOTUS 123 / DOS 6.2, M.S. Office 97/2000, VC ++ 
  

1. G. Seema, Pande H.P, Lal J and Madan V.K. (2001) Plantlet regeneration of sugarcane varieties and transient GUS expression in calli by electroporation. Sugar tech 3 (182) : 27-33
   
2. Srivastava Sangeeta and Srivastava H.M. (1988). Cytomorphology of diploid and tetraploid beets. In: International Crop Network Series, IPGRI, Rome (International Beta Genetic Resources Network) Pp. 96-99. 
   
3. Srivastava Sangeeta and Srivastava H.M. (2000). Cytological and Karyotypic studies in four species of genus Beta. Journal of Sugarbeet Research (America): 37 (4) 135-142.
   
4. Suman A., Rewari ,R.B., Kumar, S and Khanuja, SPS (1995) Heat shock tolerant mutants of Rhizobium meliloti forming ineffective nodules. J Plant Biochem & Biotechnol, 4: 5-9. 
   
5. Suman A, Shahi, H.N., Srivastava, T.K., Rai, R.K. and Gaur, A. (2000) Rhizosphere evaluation for soil quality status in sugarcane-black gram intercropping. Indian J Sugarcane Technology 15:7-13. 
   
6. Suman A, Shasany, A.K., Singh, M., Shahi, H.N., Gaur, A. and Khanuja, SPS. (2001) Molecular assessment of diversity among endophytic diazotrophs isolated from subtropical Indian sugarcane. World J Microbiol & Biotechnol, 17:39-45. 
   
7. Suman A, Shahi, H.N., Singh, P. and Gaur, A. (2002) Allelopathic influence of Vigna mungo (black gram) seeds on germination and radical growth of crop plants. Plant Growth and Regulation (In Press).
   
8. Suman A, Soloman, S, Yadav, D.V., Gaur, A and Singh, M. (2002) Post harvest loss in sugarcane quality due to endophytic microorganisms. Sugar Tech, 2:21-25. 
   
9. V.K. Madan, Bikash Mandal, S.R. Misra, M.I. Ansari, Anjani Srivastava and V.P. Aganihotri; (1997) ; Isolation and characterization of fotol and plasmid DNA from Colletotrichum falctum causing red rot of sugarcane, Sugarcane. (U.K), 6,9,-11. 
   
10. V.K. Madan, Bikash Mandal, S.R. Misra, M.I. Ansari, Anjani Srivastava, N. Soni, S. Solomon and V.P. Aganihotri; (2000); RAPD-PCR analysis of molecular variability in the red rot pathogen (Collectotrichum falcatum). Sugarcane (international); March, 2000; 5-8.

• Commercialized - Micropropagation for rapid seed multiplication and varietal rejuvenation.
• Developed - Biofertilizer, Somaclonal Variants , A protocol for transformation of sugarcane employing electroporation.
• Being Developed - Transgenics for tolerance to insect - pests involving Agrobacterium mediated transformation of sugarcane.