• HRD Overview
• Training Programme for Students
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  Babasaheb Bhimrao Ambedkar University,
  Lucknow
• UP Skill Development Mission
  Sponsored Programme
• Vocational Training

Course No. BTP01

Duration: 6 month with a minimum of 90 working days.

Eligibility:

1. B.Sc/ M.Sc in Biotechnology /Life Sciences / B.Pharma/ M.Pharma /M.B.B.S/Agriculture or veterinary Sciences
2. B.E/ B.Tech in biotechnology /Chemical engineering

The candidates should have at least 50% marks in any of the above degree

Mode of training: The training will consist of lectures; delivered by competent resource persons and practical demonstrations and a project work.
Examinations:  There will be written examinations at the end of the course. The project dissertation will be evaluated by the internal and external experts.
Maximum: 24 credits

OBJECTIVES          :

• To empower the B.Sc. / M.Sc. &B.E./ B.Tech/ M.Tech  students with the necessary skills to make them employable in biotech industries both in research and service areas. Major thrust areas for the Finishing School would be Agri- and Biopharma- industries.
• To provide students hands on training at various industrial units/ facilities of Biotech Park e.g. biofertilizer/ bio-pesticides, plant tissue culture, diagnostics & drug delivery systems, distillation & solvent extraction plant for high value products based on phytomolecules and bio-active fractions (Students would also be visiting the CSIR / ICAR laboratories in the city depending upon their subject area).
• To educate the students with the concepts of good laboratory practices, good manufacturing practices, IPR, bioethics, communication skills and regulatory requirements regarding toxicity testing, setting up of new units through lectures and practicals.

NEED FOR STARTING AFINISHING SCHOOL

Biotechnology is a sunrise sector in life sciences both in academia and industry. It is a frontier of science offering enormous possibilities of its use as a premium precision tool for the welfare of society and creation of wealth for sustainable commercial and socio-economical development. Biotechnology draws on expertise from a number of scientific fields such as Molecular and Cell Biology, Botany, Biochemistry, Microbiology, Chemical Engineering and Chemistry. Its application in health, agriculture and energy sectors have been path breaking and yielded significant results. The Biotech industry has touched the highest revenue generation of $ 4 billion in 2010-2011. With innovative programme supported by Department of Biotechnology, Government of India, the industrial growth is expected to rapidly increase in coming years.

A large number of students have been attracted to the discipline of biotechnology and a number of students are passing out each year with B.Sc/ M.Sc, B.Tech/ M.Tech degrees. Biotechnology industry needs skilled manpower for the R&D, production and services. However, majority of the students are unable to get placements as they lack skills needed by the biotech industry. Also, biotech industry on several occasions has stated the shortage of skilled manpower commensurate to their requirements. Hence, there is an urgent need to train the B.Sc., B.Tech and M.Sc., M.Tech pass outs keen to work in biotech industry, with skills to make them employable. Since the students have basic background, additional skills can be imparted through theoretical and hands on exposure in a short period of six months.

Biotech Park, a technology incubator, has setup a biofertilizer/ biopesticides and a tissue culture facility along with macro propagation facilities. The biofertilizer and tissue culture facilities are being run in partnership with industries. Biotech Park has also setup an aqueous and solvent extraction plant for obtaining essential oils and bioactive chemicals from medicinal, aromatic and other plants which are used for preparing nutraceuticals, pharmaceuticals and cosmetics. Since its inception, Biotech Park has linkages with Central Drug Research Institute (CDRI), Central Institute of Medicinal and Aromatic Plants (CIMAP), Indian Institute of Toxicology Research (IITR), National Botanical Research Institute (NBRI), Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS) and Chhatrapati Shahuji Maharaj Medical University (CSMMU) which are centers of excellence in their fields and have state-of-art facility for clinical research, drug development, toxicity testing, product formulations and development from aromatic and medicinal plants, etc. These facilities will serve as a strong base for the practical training of the students of the proposed Finishing School. Biotech Park has 3-4 lectures and meeting rooms and a Bioinformatics centre with library facilities. Biotech Park has been regularly conducting workshops in Bioinformatics and summer training programmes.

COURSE CONTENT

The course will be of six months duration where 2 months would be devoted to class room lectures and 4 months to hands on training. It will be run in collaboration with Babasaheb Bhimrao Ambedkar (Central) University (BBAU), Lucknow. During 4 months hands on training, the students will work on a project leading to their dissertation. In the end they will submit the report in bound form which will be evaluated by external experts. The students will be required to appear in the examination at the end of the training. The course contents would be developed jointly and all practical training and class room lectures will be held at Biotech Park. The students will visit centers of excellence in city like SGPGI, CDRI, IITR and others for specialized training for 3-5 days whenever required.  

Subject Areas:

The students will have a choice to select one subject area from the following:

• Bioinformatics & Rational Drug Design
• Molecular Biology: Tools and Techniques
• Microbiology: Product Development and Bioprocessing
• Nutraceuticals and Cosmeceuticals
• Plant Tissue Culture and Micro & Macro-Propagation
• Food Biotechnology: Bio-process & Quality analysis
• Pharmaceuticals: Quality Control and Formulation
• Environmental Monitoring & Bio-remediation
• Quality Control of Biotech Products

The following shall be compulsory subjects for all students:

• Documentation / Data Analysis and Communication Skills (report and paper writing and presentations)
• Concept of Good Manufacturing Practices (GMP), Good Laboratory Practices (GLP), accreditation and Intellectual Property Right (IPR).
• Bioethics , regulatory laws
• Introduction to basic microbiology,molecular biology and analytical techniques and their applications.
• Scientific writing

BIOINFORMATICS

Introduction: An overview of bioinformatics and their major resources such as NCBI, EBI, ExPASy, RCSB. The knowledge of various databases and bioinformatics tools available at these resources, data contents and formats, purpose and utility in biological research

Biological databases: Sequence database, 3D Structure Database, Derived Databases
Sequence Analysis: Basic concepts: Sequence similarity, identity and homology, definitions of homologues, orthologues, paralogues, Pairwise sequence alignments, Multiple sequence alignments (MSA)

Molecular modeling: methods and techniques
Rational Drug Design and Discovery: Role of Bioinformatics in rational drug design, Target identification and validation and lead optimization,

Chemo-informatics: Different systems for representing chemical structure of small molecules like SMILES, pdb and sdf etc, Generation of 2D coordinates of small molecules,

ADMET: In silico prediction ADMET properties for Drug Molecules, Structure based drug design: Identification and Analysis of Binding sites and virtual screening, molecular docking and their softwares, pharmacophore and qualitative structure activity relationship (QSAR).

BIOSEPARATION PROCESSES

Introduction: An overview of bioseparation, Separation of cells and other insolubles from fermented broth. Substrate processing, Medium preparation, Statistical optimization techniques.

Cell disruption: Physical, chemical and enzymatic methods

Product isolation: Extraction and adsorption method, solid-liquid separation, liquid- lquid separation, distillation, Precipitation method using ammonium sulfate, organic solvents, high molecular weight polymers, Reverse osmosis. Liquid-liquid extraction of antibiotics and Biopolymers.Recovery Processes of dextran, penicillin, citric acid and ethanol.

Filtration, mirofiltration, Dialysis, ultrafiltration. Centrifugation, coagulation, flocculation.

Electrophoresis: PAGE and agarose gel, Isoelectric focusing.
Chromatography: principles for product purification, chromatographic techniques, column, ion exchange, affinity, GLC, HPLC.
Immobilization techniques.

APPLIED BIOTECHNOLOGY

Transgenic Plants in Crop Protection: Herbicide tolerant plants, disease resistant plants, insect tolerant plants; Biological Control Methods, control of insects & other pests.

Animal Biotechnology and Cell Culture: Techniques of cell and tissue Culture: Primary culture, Cell lines and cloning, Somatic cell fusion, tissue cultures, organ culture, whole embryo culture.

Transgenic animal technology-Integration of microinjected sequences into the embryo. Specific applications of Transgenic mice, use of transgenic animals, production of transgenic sheep, Methods for the introduction of recombinant DNA into cells and embryos,  gene transfer and its methods.

Food Biotechnology: Introduction, Sterilization and pasteurization of food products. Production of bread, cheese, vinegar etc. Food preservation.

Modern applications; Genetically Manipulated Organisms and products: Uses in process biotechnology, GM Foods and current global situation, pharmaceuticals,
Industrial safety: Rules and regulations.

Biotech industries in India and abroad: Current status and potentials. Status and future in other countries.

Biopharming; Biotechnology in developed and developing countries: Current applications, regulatory issues, patent issues, trade;

Biotechnology and Information Technology (BT & IT) interdependence: need of latest information, IT applications in high-tech industry;

BIOPROCESS ENGINEERING AND TECHNOLOGY

Isolation, preservation and maintenance of industrial microorganisms

Kinetic of growth.

Introduction to bioprocess engineering, Design and types of bioreactors

Media for Industrial fermentation: Air and media sterilization, Types of fermentation, Process analysis of bioreactors, Stability of microbial reactor.

Measurement and control of bioprocess parameters.
Down stream processing: Introduction to removal of microbial cells and solid matter (Foam separation, precipitation, filtration, centrifugation, cell disruption, liquid – liquid extraction, chromatography, membrane process, drying and crystallization.

Treatment and disposal of effluents, Whole cell immobilization and their application: Industrial production of chemicals, alcohol, acids (citric, acetic, gluconic).
Industrial production of Antibiotics such as penicillin, streptomycin, tetracycline.
Industrial production of Amino acids such as Lysine, Glutamic acid etc.

DISSERTATION / PRACTICAL TRAINING

• Plant Tissue Culture – practical experiences at industrial scale for production of plants by micro and macro- propagation methods.(Plant Tissue Culture facility of Biotech Park)
• Biofertilizer unit – Industrial experience in production of bacterial biofertilizer, vermicompost; biopesticides (like Tricoderma, etc.)(Biofertilizer unit of Biotech Park)
• Extraction and Distillation Plant – Industrial experience of using solvent extraction plant and aqueous distillation plant for obtaining bioactive substances for production / formulation of cosmetics, nutraceuticals and pharmaceuticals.(Extraction and Distillation Plant of Biotech Park)
• Animal Maintenance-  All aspects of toxicity testing as per GLP requirements (CDRI / IITR)
• Drug discovery and Drug Development (CDRI)

Project work: The project work shall be based on the course content of the syllabus and the topic of the project work shall be decided based on the need of the student and the available expertise.